Informatics Publishing Limited

S. J. Daharwal ¹, Veena D. Singh ¹

Affiliations
1 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh, IN

Abstract

A new simple spectrophotometric method was developed for the determination of binary mixtures of Candesartan cilexetil (CAND) and Hydrochalorothiazide (HCTZ), without prior separation. The method is based on the multi-wavelength technique i.e. classical least square (CLS) method. The tablet is determined by the multi-wavelength technique (CLS), at the wavelengths range of 215-225 nm over the concentration ranges of 2.5-50 and 1-30 μg/mL with mean recovery more than 98% for both drugs CAND and HCTZ, respectively. The proposed spectrophotometric method was validated and successfully applied for the assay of drug combination in several laboratory-prepared mixtures and commercial tablets.

Keywords

Spectrophotometric Analysis, Classical Least Square Method, Candesartan, Hydrochlorothiazide.

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S. J. Daharwal ¹, Veena D. Singh ¹

Affiliations
1 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh, IN

Abstract

Telmisartan hydrochloride (TELM), Amlodipine besylate (AMLO), and Hydrochlorothiazide (HCTZ) are coformulated in a single-dose combination for the treatment of hypertensive patients whose blood pressure is not adequately controlled on either component monotherapy. In this work, chemometric method, classical least squares (CLS) was applied for simultaneous Spectrophotometric determination of TELM, AML and HCTZ in their combined pharmaceutical tablets. The optimum assay conditions were established and the proposed method was successfully applied for the assay of the ternary mixtures of drugs and combined pharmaceutical tablets with excellent recoveries. No interference was observed from common pharmaceutical additives. The results were favorably compared with those obtained by a reference HPLC method.

Keywords

Telmisartan Hydrochloride, Amlodipine Besylate, Hydrochlorothiazide Spectrophotometry, Chemometric Method, Pharmaceutical Tablets.

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S. J. Daharwa ¹, Veena D. Singh ¹

Affiliations
1 University Institute of Pharmacy, Pt. Ravishankar Shukla University, Raipur-492010, Chhattisgarh, IN

Abstract

Chemo Metrics, Hydrochlorothiazide, Classical least Square And Captopril.Chemo metric method was developed for the simultaneous determination of Captopril (CAPT) and Hydrochlorothiazide (HCTZ). The chemo metric method, classical least square (CLS) was successfully applied to quantify the two drugs in the mixture using the information included in the UV absorption spectra of appropriate solutions in the range of 215-275 nm with the intervals Δλ =5nm. The calibration of CLS models was evaluated by internal validation (prediction of compounds in its own designed training set of calibration) and by external validation over laboratory prepared mixtures and pharmaceutical preparations. The CLS methods require neither any separation step, nor any prior graphical treatment of the overlapping spectra of the two drugs in a mixture. The result of CLS method was compared with each other and a good agreement was found.

Keywords

Chemo Metrics, Hydrochlorothiazide, Classical least Square And Captopril.

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